International Journal of Scientific & Engineering Research, 4(11): 1257-1271 (Nov 2013)
Flower pigment analysis of Mirabilis jalapa

Sujata Mahapatra* and Bhaskar Padhy
*Lecturer in Botany, Sc. College, Hinjilicut, Ganjam, Odisha, India,

ABSTRACT
The betacyanin pigments were extracted from the Mirabilis jalapa (dark pink) flowers, of Nyctaginaceae family, available in Berhampur University campus of south eastern Odisha, using aquash acidified methanol (0.01 % Hcl).The extracted pigments were exposed to different pH. (1,4,7 ,10) at different temperature (4ºC,25ºC,35 ºC & 45 ºC) under dark conditions. The result showed that increase in pH, temperature or exposure to light destruct the betacyanin pigments. The antioxidant activity of methanolic extract of the test flower and the standard antioxidant ascorbic acid was assessed on the basis of the radical scavenging effect of the stable 2, 2- diphenyl-1-picryl hydrazyl (DPPH) free radical activity according to the method described by (Sadhu et al., 2003).

The betalain are yellow-to-red nitrogen-containing compounds, derived from the amino acid tyrosine. It comprises the largest group of water soluble pigments in the plant kingdom, only in the order Caryophyllalles giving the natural colour yellow, magenta or dark pink to the flower, fruits and coloured leaves. They are included in flavonoids or phenolic compounds (Malian-aubert et al, 2001).They are synthesized in the plant cell cytoplast and actively transported across the tonoplast into the vacuole, where they accumulate (leng et al, 2000).Betalains are classified into red (crimson) betacyanins and yellow betaxanthins (Strack et al., 2003)

Mirabilis Bibliography