Propagation of Ornamental Plants 12(2): 109-116 (2012)
THE INFLUENCE OF TIBA ON ADVENTITIOUS SHOOT INDUCTION OF ROSE CULTIVARS
Danuta Kucharska, Teresa Orlikowska, Anna Stępowska, and Barbara Dyki
Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice Poland,

Abstract
In our experiments on enhancement of in vitro regeneration potential of rose we have found that the addition of 2.5 mg l-1, TIBA, to the regeneration medium containing BAP intensified regeneration of adventitious shoots on leaves of rose cultivars White Gem, Mistral, and Charming and prolonged an efficient regeneration for the third and fourth subcultures. TIBA significantly enhanced the number of responding leaves and number of adventitious shoots. Effect of TIBA was counteracted by the addition of 0.01 mg l-1 NAA to the regeneration medium. Histological analyses showed that regeneration medium containing BAP and TIBA stimulated continuous cell division and formation of meristems and buds whereas on the medium without TIBA less of initiated meristems and differentiating buds but xylem clusters were observed.


2,3,5-Triiodobenzoic Acid is an inhibitor of polar auxin transport and more commonly known as TIBA.

6-Benzylaminopurine, benzyl adenine, BAP or BA is a first generation cytokinin plant growth regulator influencing plant growth and development, setting blossoms and stimulating fruit richness by stimulating cell division.


Vegetative Selection

Adventitious Shoots