Biochem J.  1944; 38(3): 279-282.
Carotene and Lycopene in Rose Hips and Other Fruits
By F. C. JACOBY AND F. WOKES,
Ovaltine Research Laboratories, King's Langley, Herts
(Received 18 May 1944)

Rose hips have been previously reported to contain carotene (Kuhn & Grundmann, 1934; Wokes, Johnson, Organ & Jacoby, 1942) and lycopene (Karrer & Widmer, 1928; Escher, 1928), and they have also been found to possess considerable vitamin A activity (Svensson, 1936). Sufficient care has not always been taken to differentiate between the various carotenoid pigments. Moreover, data are needed on the amounts found in different species.

This paper provides such data by describing a simple method of estimating carotene and lycopene separately in the presence of other pigments, which has also given satisfactory results when applied to tomatoes and Solanum Dulcamara berries. The results on dried rose hip extract have been checked by a biological assay confirming the presence of vitamin A activity.

METHODS

Materials. Ripe rose hips of known species collected in the Durham area during October and November 1943 by Prof. Heslop Harrison, and in the Royal Botanic Gardens, Kew, during September 1943 by Dr R. Melville were posted immediately to King's Langley and stored in dry bottles in a refrigerator until examined; tests at intervals during the experimental period showed no loss of carotenoids under the given storage conditions. The samples of dehydrated rose hips were taken from large-scale batches prepared in the Ovaltine Laboratories in 1942-4 from locally grown hips, mainly Rosa canina and R. dumetorum. Ripe Solanum Dulcamara berries were collected in the King's Langley area during October 1943 and examined immediately. Ripe tomatoes grown out of doors in King's Langley in 1943 were preserved in jars by the usual method and when opened for examination 3 months later were in excellent condition.

Extraction of carotenoids. For this we have used an excellent unpublished method for extracting carotene from plant material devised by Dr Vernon Booth, to whom we are greatly indebted for advice and help. It was applied to rose hips as follows:

About 5 g. typical rose hips were selected and weighed. The flesh was dissected from seeds, stalks, etc., divided into two portions and weighed. Tests showed that no significant loss of moisture took place during the dissection, so that results with the flesh could be correlated with those from whole fruits. Each portion of the flesh (c. 1 g.) was ground with quartz powder and a mixture of acetone and petrol ether (2:3 by volume). The supernatant yellow solution was decanted into a separating funnel and the residue ground with more acetone-petrol ether mixture until no more colour was extracted. (With dried extracts and other dry material a few drops of distilled water were added to facilitate extraction.) The combined extracts were then washed with distilled water by a continuous flow apparatus, until the absence of striae as the drops of water fell through indicated that all acetone had been removed. Usually about a litre of water, passing through at the rate of about 2 drops per sec., was required to remove the acetone from 50 to 100 ml. of combined extract. The xanthophylls, which usually formed less than half of the total pigments, were removed by shaking two or three times with one-third volume of diacetonol until practically no further colour was removed. The diacetonol was then washed out with water.

Chromatographic separation. The adsorbent used in this investigation was Alocol brand colloidal aluminium hydroxide (A. Wander Ltd, King's Langley) activated by heating at 1000 for a few minutes immediately before use. Its watery suspension had pH 7.4-7.5 as compared with 65-9.5 for other brands of alumina. The average particle size determined by means of haemocytometer was 25-100µ, which is considerably higher than the 7µ found by Zechmeister & Cholnoky (1941) for Merck alumina standardized by Brookmann's method. However, comparison with Merck and several other brands of alumina in use in this country at present has shown the Alocol after activation to be as efficient as any for separating carotene and lycopene. Recovery experiments with widely varying proportions of p-carotene and lycopene also gave satisfactory results. Details of this comparison will be published later.

The petrol ether extract, prepared as above, was percolated through a column of alumina (20 x 2 cm.). The pigments were adsorbed in a zone about 1 cm. deep and about 1 cm. from the top. The chromatogram was developed with petrol ether containing 1% acetone, when the yellowish orange carotene layer quickly separated from the reddish orange lycopene layer and, moving rapidly down the column, could be completely eluted. This usually left at the top of the column a narrow yellowish band possibly due to small amounts of kryptoxanthin. The lycopene was then eluted with benzene. The separate solutions of carotene and lycopene were stabilized with nitrogen, and stored in the dark in a refrigerator, under which conditions no appreciable loss occurred during several days. Throughout the extraction and separation of the pigments oxidation was prevented by keeping them moist' with solvent and by screening them from light.

Spectrophotometric estimation of carotene and lycopene: This was done with a Hilger-Nutting constant deviation wave-length spectrophotometer calibrated for wave-length measurements against the emission spectrum of a helium lamp and for density measurements against a set of neutral glasses standardized by the National Physical Laboratory. The solutions, diluted if necessary with the respective solvents, were placed in 10 or 20 mm. cells of Hilger's type D, which can be readily filled and stoppered without inclusion of air bubbles in the body of the cell. The mean density was determined at 450 mµ for carotene in petrol ether (b.p. 40-60°) and 482 mµ for lycopene in benzene, using the cross-over technique of Wokes & Still (1942) which compensates for zero changes and other instrumental causes of error. Readings were taken at 10 mµ above and below the given wave-lengths to define the exact position of the maximum, and in a number of instances the whole curve was determined from 450 to 520 or 530 mµ. The results, summarized in Table 1, showed excellent agreement between carotene and lycopene separated from different sources and specimens of the pure pigments examined by ourselves and other workers.

Table 1: Absorption data for carotene and lycopene

  -carotene Lycopene
Solvent max (mµ) max (mµ)
Petrol ether 450 2500 474* 2425
Benzene 463 2250 482 2025
Chloroform 463 2200* 480* 2000*
* Morton (1942).

Calculation of results. When calculating the concentration of carotene and of lycopene in our solutions we used the values given in Table1. The value of 2025 for lycopene 1 cm. in benzene was determined as follows. Lycopene was extracted from Sciences Dulcamara berries by the above method, and separated by chromatography from carotene and all other pigments. Solutions of the purified lycopene were prepared in chloroform, benzene and petrol ether (b.p. 40-60°) and their respective densities determined. From these densities were calculated the values for in benzene and petrol ether, the value given by Morton (1942) for lycopene in chloroform being used as a basis. The benzene solution of the lycopene gave max. 482 mµ (between max. 486mµ found by Zechmeister & Tuzson (1938) and max. 480mµ found by Sivadjian (1938)) and the petrol ether solution gave max. 473 mµ, in good agreement with max. 474 mµ (Morton, 1942). The same technique applied to -carotene in benzene gave of 2250 at max. 463mµ., differing by less than 2% from the value of 2280 at max. 464 mµ (Morton, 1943). Hence the error introduced by using the value 2025 for lycopene was probably less than 2%, of which only part could be attributed to the difference of 2-4 mµ in position of maxima as observed by ourselves and other workers, since the absorption curves for lycopene and carotene in Fig. 1 show fairly broad peaks at the above maxima.

Wave-length of light (mµ)

Fig. 1. Absorption curves for -carotene (specimen also tested by R. A. Morton) and for lycopene extracted from Solanum Dulcamara berries, -carotene in petrol ether + 1 % acetone o——o. Lycopene in benzene x——x.

Sampling errors. These form the chief part of the experimental error. The average coefficient of variation for estimations on separate hips was 36. This was reduced to 15.7 by bulking 5 g. of hips comprising 3-12 hips, which after removal of seeds, etc., yielded 2-3 g. flesh. The. average coefficient of variation between different 1 g. samples on the same lot of flesh from 5 g. portions of hips was only 5.1, showing that the method of preparing the samples had produced a much more uniform material than the original rose hip population. However, when dehydrated extracts of rose hips were made, the coefficient of variation between single assays on this material was still lower, showing that still greater uniformity had been obtained. Assuming that the experimental error was responsible for a coefficient of variation of not more than 3%, and deducting this from the average coefficient of variation of 36 for separate hips, there is left at least 33% as a measure of the variation to be expected from one rose hip to another.

RESULTS

Known species of roes hips. These are summarized in Tables 2 and 3 in order of decreasing carotene content. Each result is the mean of at least two assays in which the average error was as described above. The carotene content of the Durham samples was 74-187 µg./g., and of the Kew samples 41-671 µg./g. The lycopene content was usually higher than the carotene content, except in one or two species. The ratio between carotene and lycopene in a given species did not vary in duplicates by more than experimental error. The result of 187±6 pg./g. for carotene in the hybrid of Rosa mollis and R. dumetorum was significantly higher than the results of 74±1 for R. dumetorum hips collected at the same time in the same area, and higher than the result of 83± 50 for R. mollis hips from Kew, although not much higher than the result of 161±11.2 for R. dumetorum var. Gabrielis hips from the Durham area. It should be noted that the hybrid was almost sterile, yielding less than a dozen hips on the whole bush.

Table 2. Carotene and lycopene in flesh of fresh rose hips from Durham

  Carotene
(µg./g.)
Lycopene
(µg./g.)
Hybrid between R. dumetorum and R. mollis 187 276
R. dumetorum var. Gabrielis 161 232
R. dumalis var. Reuteri 149 532
R. dumaiis var. subcristata 148 834
R. canina var. sarmentacea 146 268
R. tomentosa var. scabriuscula 142 141
R. obtusifolia var. sclerophylla 119 314
R. dumalis var. subcanina 118 229
R. coriifolia var. frutetorum 113 246
R. canina var. vinacea 98 223
R. dumetorum var. pseudoincerta 74 104

Note. We are informed by Prof. Heslop Harrison that in following the International Rules of Botanical Nomenclature, R. dumalis is the correct name to use for the species described as R. glauca or R. Afzeliana in Wolley-Dod's Revision the British Roses (1930-1), the varietal names being the same except for var. subcristata, which is var. glaucophylla W.-Dod. The use of R. dumalis in this sense necessitates the change of R. canina var. dumalis to R. canina var. sarmentacea.

Table 3. Carotene and lycopene in flesh of fresh rose hips from Kew

  Carotene
(µg./g.)
Lycopene
(µg./g.)
R. centifolia 671 206
R. Davidii 242 657
R. carolina var. Nuttalliana 233 598
R. virginiana 230 327
R. moschata 210 147
R. calocarpa 175 150
R. Wilsoni 169 367
R. agrestis 123 537
R. Helenae 108 94
R. canina var. stenocarpa 100 204
R. Sabini 93 238
R. rubiginosa 74 595
R. mollis 63 283
R. spinosissima 50 146
R. coriifolia 41 354

Effect of latitude. Pyke & Melville (1942) found the vitamin C content of rose hips to be related to the latitude in which they are indigenous, the higher contents being found in higher latitudes. We did not find any positive relation between carotene content and latitude in native species showing marked differences in their vitamin C content (see Table 4). In fact our data suggested a negative correlation but were insufficient to establish this significantly.

Table 4. Distribution of rose hips and their vitamin A and C values

  Vitamin C*
(mg./100 g.)
Carotene
(µg./g.)
R.  mollis 1260 63
R. coriifolia 1080 77
R. rubiginosa 810 74
R.  tomentosa 690 142
R. dumetorum 590 118
R. canina 550 123
R. agrestis 460 123
R. obtusifolia 420 119
R. spinosissima 340 50
* Pyke & Melville (1942).

Comparison of carotene content with vitamin A activity. For this comparison it was thought desirable to obtain material of greater stability and more uniform composition than could be found in fresh rose hips. Dried extracts were therefore prepared from-fresh ripe hips by extraction with boiling water and evaporation of the extract in vacuo, with precautions to avoid loss by oxidation. Tests showed that not more than 20% of the vitamin C had been lost during the preparation of the extracts, which were found to be rich sources of vitamins C and P as well as containing considerable amounts of carotene. A biological assay of a sample of one of these dried extracts, after it had been stored about 9 months in powdered form in air at room temperature, was carried out in the Pharmaceutical Society's laboratories and showed it to have vitamin A activity equivalent to 44 i.u./g. (23-86 for P=0.95). Chemical estimation of the carotene content before and after the biological test showed a gradual loss of carotene (from 45 to 16 i.u./g. in 14 months) and the carotene content at the time of the biological test was only about 60% of the amount shown by the biological test, if one assumes that 1 µg. carotene = 166 i.u. vitamin A. This rate of loss can be greatly reduced by storing the extracts in the form of compressed tablets. Taking into consideration the error of the biological method as well as other possible sources of error, this difference was not highly significant. However, since the biological result was higher than expected on the chemical assay indicated, it was clear that the carotene estimation did not provide too high an indication of the. vitamin A value, and that the chromatographic separation of lycopene from carotene was complete.

Results on other materials. The method, having given satisfactory results with rose hips in which the L/C (lycopene/carotene) ratio ranged from 03 to 87, was then applied to other materials in which this ratio varied still more widely, e.g. Solanum Dulcamara berries with L/C ratio of 17.2, dehydrated carrots rich in carotene and containing practically no lycopene, and tomatoes, which have in the past been given exaggerated vitamin A values because of failure to differentiate efficiently between carotene and lycopene. The results, summarized in Table 5, showed satisfactory separation of the carotene from the lycopene and were on the whole in good agreement with those obtained by other workers who also separated the two pigments satisfactorily (Kuhn & Grundmann, 1934).

Table 5. Carotene and lycopene contents of different materials

  Carotene
(µg./g.)
Lycopene
(µg./g.)
R. centifolia hips 671 206
Kew rose hips, 15 varieties, mean 172 327
Durham rose hips, 11 varieties, mean 132 309
Tomato skins, cooked, mean 73 416
R. coriifolia hips 41 354
Tomato flesh 7 33
Solanum Dulcamara berries 6 103
Results by other workers:    
Kuhn & Grundmann (1934):    
Tomatoes 7 79
R. canina hips 255 160
R. rubiginosa hips 230 275
Smith (1936):    
Tomato skins 2652-8862  

In the skins of cooked tomatoes the carotene content of a number of samples was very much lower than that found by Smith (1936) in the skins of American tomatoes. The lycopene/carotene ratio in the skins was, however, similar to that found in tomato flesh by ourselves and by Kuhn & Grundmann (1934).

DISCUSSION

The estimation of carotene in materials containing lycopene, presents difficulties. Failure to achieve efficient separation may lead to exaggerated claims for the vitamin A value of certain foods (e.g. tomatoes) if these claims are based on carotene estimations. Moreover, during the extraction of the pigments changes may occur through isomerization or oxidation. These difficulties have been overcome by using (a) extraction methods avoiding the use of heat and requiring minimum of exposure to air and light; (b) chromatographic separation with a specially prepared adsorbent of proved efficiency; (c) immediate spectrophotometric estimation of the pigments.

These precautions have enabled us to obtain reasonably accurate results on the different species of rose hips as shown by the coefficient of variation of 5-1 for different samples from the same batch of minced rose hip flesh.

It should be emphasized that this degree of accuracy is applicable only to the experimental material as we obtained it, and does not take into consideration variations caused by habitat, climate and other factors. However, since the average figure for all the-Kew samples varied by lees than 30% from the average figure for all the Durham samples, the differences in conditions in these two areas could not account for the much wider variation in carotene and lycopene content in different species. Moreover, in a series of dried extracts made mainly from Rosa canina and R. dumetorum hips collected in the King's Langley area, there was less than 30% deviation from one batch to another and from 1942 to 1943 seasons. Hence. there in little doubt that ripe rose hips in general contain considerable amounts of carotene, and that some species contain much more than others.

SUMMARY

  1. A method is, described for extracting, separating, and estimating carotene and lycopene from plant materials, which minimizes isomerization and oxidative changes.
  2. Applied to the flesh of ripe rose hips, this method showed carotene contents of 41-671 µg./g. and lycopene contents of 94-834 pg./g. in 26 distinct species and hybrids.
  3. The method also gave satisfactory results with dried rose hip extracts, Solanum Dulcamara berries, tomatoes and other sources of carotenoids. The vitamin A value of a sample of dried rose hip extract as determined by biological assay was at least as high as was indicated by our chemical estimations of the carotene.

We are indebted to Prof. J. W. Heslop Harrison and Dr R. Melville for specimens of known species of rose hips, to Mr A. L. Bacharach for a specimen of -carotene, to Drs R. A. Morton and Vernon Booth for advice and criticism, and to Miss Valerie Pritchard for assistance.

REFERENCES